Researcher: John Eric W. David, April 10, 2018 Adviser: Niel P. Rigdao
Abstract
The Kingdom Plantae represents a vast supply of secondary metabolisms with different chemical structures to prevent diseases, but not all secondary metabolites are good to the health some have toxicity potentials. Balakat tree (Ziziphus talanai (blanco) merr.). Is endemic in the Philippines. The Balakat tree consist a thorny bark the and it possessed an hepatoprotective potentials, Phytochemical screening using Thin Layer Chromatography determined that aqueous leaf extract of balakat tree have Essential oils, Triterpenes, Phenols, Steroids, Coumarines, Tannins, Flavonoids, and Alkaloids, while the secondary metabolites present in the ethanol leaf extract are Essential oils, Triterpenes, Steroids, Phenols, Anthraquinones, Coumarines, Anthrones, Tannins, Flavonoids and Alkaloids. Brine Shrimp lethality assay and Zebra fish lethality assay was used to determined cytotoxicity potentials and teratogenic effects. All the extract which is aqueous and ethanol produced different dosages (1ppm, 10ppm, 100ppm, 1000ppm) of leaf extract exhibiting none toxic to the nauplii and zebra fish embryo, teratogenic effect was observed within the both extract which is delayed development that is noted under 10ppm that has 50% of hatchability percentage and 50% delayed development, 100ppm presents percentage and a 50% hatchability 50% delayed development, and 1000ppm presents 25% hatchability percentage and 75% delayed development of aqueous extract while in ethanol extract10ppm presents 75% of hatchability percentage and 25% delayed development, 100ppm presents 0% hatchability percentage and a 100% delayed development, presents 0% hatchability percentage development. and 1000ppm and 100% delayed
Keywords: Ailments, Monoecious, Thin Layer Chromatography, Coumarines, Anthrones, Anthraquinones, Teratogenic
David, J. E. W. (2018, April 10). Phytochemical screening, comparative cytotoxicity and teratogenic potentials of aqueous and ethanol leaf extract of Balakat tree (Ziziphus talanai (Blanco) Merr.) using lethality assay [Undergraduate thesis, Mabalacat City College].