The Study Area

The research was conducted across 27 barangays of Mabalacat City, Pampanga, located at approximately 15°13′N, 120°34′E. Known for its rich soil and tropical monsoon climate, the area supports diverse plant life, including the culturally significant Balakat tree. Recent deforestation trends emphasize the need for conservation efforts and better biodiversity monitoring.

Sampling Techniques

Three sampling methods were used:

  • Purposive Sampling for selecting participants involved in the system testing.
  • Census Sampling for the complete inventory of all existing Balakat trees.
  • Convenience Sampling for Alpha and Beta testing users.

Plant Collection

Young Balakat leaves were collected from areas not covered in the previous tree inventory. Permission was secured for collections done on private properties. Tools used included gloves, secateurs, and plastic bags.

Preservation and Identification
Collected leaves were preserved through pressing and drying, following standard herbarium practices. Identification was done using online databases and comparison with previous studies. For scientific verification, specimens were submitted to the Jose Vera Santos Memorial Herbarium at UP Diliman, labeled with full collection details.

Geographical Coordinates and Population Study of the Balakat Trees in Mabalacat City

Mapping the Coordinates of the Balakat Trees
To map the geographical coordinates of the Balakat trees within Mabalacat City, we used the GPS Map Camera and DA Geo Camera mobile application. This user-friendly app was selected for its portability, compatibility with both Android and iOS platforms, and its ability to record GPS coordinates directly into photos. During our fieldwork, each Balakat tree we encountered was photographed using the app, which automatically captured the latitude, longitude, date, and time of the photo. These geo-tagged images were then stored in our phone gallery for documentation and analysis.

Population Density Calculation

For calculating the population density of Balakat trees in Mabalacat City, we applied the ecological formula:

Formula Where

Dp – is the population density
N – is the total number of Balakat trees recorded
A – is the area (in square meters or square kilometers)

We counted the number of Balakat trees present within each identified area from our GPS mapping, then calculated the density by dividing that number by the corresponding land area. This provided insight into how densely Balakat trees are distributed across different zones of the city.

Relative Frequency Calculation

To analyze how often Balakat trees occurred in each surveyed location, we computed the relative frequency using the formula:

Formula Where

RF – is the relative frequency
f – is the number of Balakat trees in a specific area
n – is the total number of trees recorded across all areas

This approach allowed us to understand the distribution pattern of the species by identifying which barangays or locations had higher or lower concentrations of Balakat trees.

Morphometric Data and Carbon Sequestration Measurement of the Balakat Trees in Mabalacat City

Height Measurement

We measured the height of each Balakat tree using a clinometer. Standing at a fixed horizontal distance from the base of the tree, we used the clinometer to estimate the angle from the ground to the top and bottom of the tree. By applying the formula:
we obtained the approximate vertical height of each tree. This method allowed us to calculate height efficiently, even for taller trees.

Diameter Measurement

To get the diameter, we used a measuring tape to wrap around the trunk of the tree at 4.5 feet (or approximately 1.37 meters) from the ground. This gave us the circumference, which we then converted to diameter using standard calculations. The measurements were recorded in centimeters, ensuring consistency for further computations.

Carbon Sequestration Calculation

Once we had both diameter (D) and height (H), we followed a step-by-step method based on formulas from Fransen (2024) to estimate the carbon sequestration potential of each tree.

Above-Ground Biomass (AGB):
AGB = 0.25 x D2 x H
Below-Ground Biomass (BGB):
BGB = 0.2 x AGB
Total Biomass (TB):
Total Biomass (TB) = AGB + BGB = AGB + 0.2 x AGB = 1.2 × AGB.
Total Dry Weight (TDW):
Total Dry Weight (TDW) = TB × 0.725
Total Carbon (TC):
Total Carbon (TC) = TDW × 0.5.
Carbon Dioxide Sequestration (CO₂ weight):
CO2 weight = TC × 3.67.

By following these computations, we were able to quantify the amount of carbon dioxide that each Balakat tree can sequester, contributing to an understanding of their role in carbon capture and environmental sustainability.

Phenotypic Assessment of Potential Mother Tree

To identify a potential mother tree, we followed the guidelines set by the Department of Natural Resources (2019), which define a mother tree as one that serves as the primary source for seeds intended for propagation. This selection process is critical to ensure that only high-quality, long-living trees are used for future regeneration efforts.

We used both objective measurements and subjective visual observations for the assessment. The diameter at breast height (DBH) was measured as a key standard, while additional parameters were observed visually. Four major criteria were assessed, each with its own grading system. These are described below:

Stem Form

Stem Straightness

We evaluated the straightness of each tree’s stem from the base up to the crown. The classification was as follows:
Each tree was observed from multiple angles to ensure consistent assessment. We assigned a numerical score based on its straightness.

Stem Forking/Branching

We checked for the presence of stem forks, focusing on whether they occurred above or below 5 meters from the ground.
Each tree was observed from multiple angles to ensure consistent assessment. We assigned a numerical score based on its straightness.
We visually compared the cross-section of the trunk to the classification standards and assigned the appropriate grade.

Stem Circularity

To assess the tree’s form and uniformity, we measured the circularity of the stem beginning from DBH and continuing at 5-meter intervals.
We visually compared the cross-section of the trunk to the classification standards and assigned the appropriate grade.

Tree Health

Your Attractive HeThe overall health of the tree was evaluated based on the crown’s condition, looking specifically for signs of:
⦁ Discoloration
⦁ Physical damage
⦁ Pest infestations
⦁ Diseasesading

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