Researcher: Winnie F. Esguerra, March 2016 Adviser: Ruiza Ismael
Angiogenesis is the process of the formation of a new blood vessel from the pre-existing blood vessels which is controlled by the different regulatory factors. Excessive and insufficient growth of blood vessels will lead to various diseases. Tremendous formation of blood vessel will lead to the progression of tumor that can cause cancer. The present study was meant to examine the effect of Ziziphus talanai on angiogenesis by using CAM Assay on duck embryo model. Fertilized eggs of Anas platyrynchos were incubated on the 3rd day at 37 Β°C and the different concentrations of extract 100%, 75%, 50% and 25% are applied on the 10th day of incubation. Blood vessel formations were counted manually on the 13th day of incubation after being exposed on the different concentrations. One Way Analysis of Variance followed by the Fishers LSD post hoc test for the pair wise comparison were used to further identify the significance of the study. Based on the observations, Z.talanai shows a potential of an antiangiogenic effect by reducing the number of the blood vessels as affected by the different concentrations. A significant difference was indicated between the negative control and the different concentrations used, it shows that the greater the concentration, the lesser the blood vessels observed and counted. Concentrated extract of Z.talanai shows the lesser blood vessel branch points mean while the 25% concentration of Z.talanai got the highest mean value of blood vessel branch points observed and counted among the concentrations used. Therefore, based on the result, Z.talanai might have the potential as an antiangiogenic agent.
Keywords: Angiogenesis, CAM Assay, Ziziphus talanai, Ethanol extraction, Cancer
Esguerra, W. F. (2016). Antiangiogenic effect of ethanol leaf extract of Ziziphus talanai using CAM assay [Unpublished undergraduate thesis]. Mabalacat City College.