Researcher: Angelyn B. Esguerra, June 2019 Adviser: Aaron Carl V. Tejano
Pain is a protective mechanism from physical suffering or discomfort caused by illness or injury while inflammation is the biochemical, structural and cellular non-specific protective process occurring in vascularized tissues that designed to destroy impairing factors. This study projected screening of medicinal plants for analgesic and anti-inflammatory activity. Phytochemical screening of the ethanol bark extract of Balakat tree (Ziziphus talanai (Blanco) Merr.), revealed the presence of bioactive compounds in the ethanol bark extract of 2.talanai such as essential oils, tripertenes, steroids, tannins, flavonoids and alkaloids, anthraquiones, anthrones, steroids, coumarines. The study involved 9 albino mice for analgesic and screening and 9 albino rats for anti-inflammatory activity. For analgesic screening, mice were induced with 0.3 ml of 1% acetic acid solution. T- sample were treated with saline solution (10 ml/kg B.W); T+ sample were treated with the standard drug paracetamol (150 mg/kg B.W); and T1 bark extract (500 mg/kg B.W) and were observed for 10 minutes. While, for anti-inflammatory activity, rats were induced with 0.1 ml of the phlogistic agent albumin. T- sample were treated with saline solution (10 ml/kg B.W); T+ sample were treated with the standard drug indomethacin (100 mg/kg B.W); and T1 sample were treated with bark extract (500 mg/kg B.W) and were observed from 0 hour to 5th hour. In terms of the percentage of inhibition, writhing countings and swelling degree of both pain and inflammation, the ethanol bark extract of Z.talanai illustrated an effective inhibitory response due to the biochemical compound flavonoids such as hesperidin, apigenin, luteolin, and quercetin against COX-1 and COX-2 vasoactive substances and prostaglandins that promotes pain and inflammation and were correlated to both standard drug paracetamol and indomethacin.
Keywords: Ziziphus talanai, Analgesic, Anti-inflammatory, Writhing, Paw edema, Swelling, Flavonoids.
Esguerra, A. B. (2019). Analgesic screening and anti-inflammatory potential of ethanol bark extract of Balakat tree (Ziziphus talanai (Blanco) Merr.) in murine and rat models [Unpublished undergraduate thesis]. Mabalacat City College.