Researcher: Mr. Alex John S. Canlas, March 15, 2017 Adviser: Mr. Adrian Miclat
Abstract
The study aimed evaluate the phytochemical screening within different solvents (Acetone, methanol, Aqueous), Cytotoxic screening and analgesic potential of balakat leaf extract (Ziziphus talanai (Blanco) Merr.) The decocted revealed the presence of compounds such tannins, alkaloids, saponins, and flavonoids. For Acetone it also shows the presence of tannins, alkaloids, saponins, and flavonoids. And the Methanol extract also has the bioactive compounds tannins, alkaloids, saponins, and flavonoids. Also there is a significant difference in the mortality rate of the brine shrimps as affected by the different doses levels namely, 1 ppm, 10 ppm, 100ppm, 1 000 ppm, 10 000 ppm concentrations in this and it is also found out that the increase of the dose level of the balakat leaf extract increases the mortality of the brine shrimp. As the length of the exposure increases, the mortality rate of the brine shrimp case also increases. Analgesic property also indicates that it has potentials to be a drug to be use as an analgesia. The Paracetamol shows the highest mean, 35.92%, 34.82% and 32.34%, Decocted 29.58%, 25.18%,27.2% second, while the acetone and methanol shows the third and fourth 29.58%, 25.93%, 24.27%, and 26.75%,24.44%, 22.06% respectively. The presence of principal alkaloids is widely used as an analgesic and sedative. One example for this is Caffeine as for stimulant and diuretic which can be found especially in coffee, tea and tobacco also for the relieve of pain and cocaine as a local anesthetic (Macatuno, M. P., 2008)
Keywords: Bio-active compounds, mortality rate, analgesia, Acetone and Methanol, Balakat Leaf extract
Canlas, A. J. S. (2017). Phytochemical and cytotoxicity screening, and analgesic potentials of Balakat leaf extract (Ziziphus talanai (Blanco) Merr.) using different solvents in Mus musculus L. [Undergraduate thesis, Mabalacat City College].